Species identfcaton and populaton structure analysis in Geranium subg. Geranium (Geraniaceae)

Somayeh Esfandani-Bozchaloyi, Masoud Sheidai, Maryam Keshavarzi, Zahra Noormohammadi


Species identifcation is fundamentally important within the felds of biology,
biogeography, ecology and conservation. Te genus Geranium L. (Geraniaceae)
comprises about 430 species distributed throughout most parts of the world.
According to the most recent treatments, subg. Geranium is the largest subgenus with over 370 species classifed in ten sections. Te subg. Geranium is represented in Iran by 13 species. Tese species are grouped 3 sections. In spite vast distribution of many Geranium species that grow in Iran, there are not any available report on their genetic diversity, mode of divergence and patterns of dispersal.
Terefore, we performed molecular (ISSR markers) and morphological studies
of 102 accessions from 13 species of Geranium (subg. Geranium) that were collected from different habitats in Iran. Te aims of present study are: 1) can ISSR markers identify Geranium species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? Te present study revealed that combination of morphological and ISSR data can identify the species.

Ključne besede

ISSR, morphology, species identifcation

Celotno besedilo:

PDF (English)


Aedo, C. & Estrella, M. D. L. 2006: Taxonomic revision of Geranium subsect. Tuberosa (Boiss.) Yeo. Israel Journal of Plant Sciences 54:19–44.

Aedo, C. 1996: Revision of Geranium subgenus Erodioidea (Geraniaceae). Systematic Botany Monographs 49:1–104.

Aedo, C., Alarcón, M. L., Aldasoro J. J. & Navarro, C. 2007: Taxonomic revision of Geranium subsect. Mediterranea (Geraniaceae). Systematic Botany 32: 93–128.

Aedo, C., Aldasoro, J. J. & Navarro, C. 1998b: Taxonomic revision of Geranium L., sections Divaricata Rouy and Batrachioidea W.D.J. Koch (Geraniaceae). Annals of the Missouri Botanical Garden 85: 594–630. DOI: 10.2307/2992018

Aedo, C., Aldasoro, J. J., Sáez L. & Navarro, C. 2003: Taxonomic revision of Geranium sect. Gracilia (Geraniaceae). Brittonia 55: 93–126.

Aedo, C., Fiz, O., Alarcón, M. L., Navarro, C. & Aldasoro J. J. 2005a: Taxonomic revision of Geranium sect. Dissecta (Geraniaceae). Systematic Botany 30: 533–558.

Aedo, C., Navarro, C. & Alarcón, M. L. 2005b: Taxonomic revision of Geranium sections Andina and Chilensia (Geraniaceae). Botanical Journal of the Linnean Society 149: 1–68.

Armbruster, W. S. 1993: Evolution of plant pollination systems: hypotheses and tests with the neotropical vine Dalechampia. Evolution 47: 1480–1505.

Baker, H. G. 1955: Self-compatibility and establishment after ‘‘longdistance’’ dispersal. Evolution 9: 347–349.

Baker, H. G. 1967: Support for Baker’s law as a rule. Evolution 21: 85–56.

Carlquist, S.H. & Bissing, D. 1976: Leaf anatomy of Hawaiian Geranium in relation by ecology and taxonomy. Biotropica 8: 248–259.

Davis, P.H. 1967: Geranium L. In: P.H. Davis, J.Cullen & J.E. Coode (eds.), Flora of Turkey, vol 2. University Press, Edinburg 19: 451–474.

Davis, P.H. 1970: Geranium sect. Tuberosa, revision and evolutionary interpretation. Israel Journal of Plant Sciences 19: 91–113.

Duminil, J. & Di Michele, M. 2009: Plant species delimitation: A comparison of morphological and molecular markers. Plant Biosystems 143: 528 –542.

Esfandani-Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. 2017a: Genetic Diversity and Morphological Variability In Geranium Purpureum Vill. (Geraniaceae) Of Iran. Genetika 49: 543–557. https://doi.org/10.2298/GENSR1702543B

Esfandani-Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. 2017b: Species Delimitation In Geranium Sect. Batrachioidea: Morphological And Molecular. Acta Botanica Hungarica 59(3–4):319–334. doi: 10.1556/034.59.2017.3-4.3

Esfandani-Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. 2017c: Genetic and morphological diversity in Geranium dissectum (Sec. Dissecta, Geraniaceae) populations. Biologia 72(10): 1121–1130. DOI: 10.1515/biolog-2017-0124

Esfandani-Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. 2017d: Analysis of genetic diversity in Geranium robertianum by ISSR markers. Phytologia Balcanica 23(2):157–166.

Evanno, G., Regnaut, S. & Goudet, J. 2005: Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology 14: 2611–2620.

Falush, D., Stephens, M. & Pritchard, J.K. 2007: Inference of population structure using multilocus genotype data: dominant markers and null alleles. Molecular Ecology Notes 7: 574–578.

Freeland, J.R., Kirk, H. & Peterson, S.D. 2011: Molecular Ecology (2nd ed). Wiley-Blackwell, UK, 449 pp.

Frichot, E., Schoville, S. D., Bouchard, G. & Francois, O. 2013: Testing for associations between loci and environmental gradients using latent factor mixed models. Molecular Biology and Evolution 30: 1687–1699.

Hammer, Ø., Harper, D.A. & Ryan, P.D. 2012: PAST: Paleontological Statistics software package for education and data analysis. Palaeontologia Electronica 4: 9.

Hedrick, P. W. 2005: A standardized genetic differentiation measure. Evolution 59:1633–1638.

Huson, D.H. & Bryant, D. 2006: Application of Phylogenetic Networks in Evolutionary Studies. Molecular Biology and Evolution 23: 254–267.

Janighorban, M. 2009: Flora of Iran. Geraniaceae. Vol. 62. Te Research Institute of Forests and Rangelands, 64 pp. [in Persian].

Jost, L. 2008: GST and its relatives do not measure differentiation. Molecular Ecology 17: 4015–4026.

Keshavarzi, M. 2015: Infrageneric classifcation of Geranium (Geraniaceae) based on fruit and seed morphology. Acta Biologica Szegediensis 59: 45–54.

Keshavarzi, M., Behzadifar, M. & Nazem Bokaee, Z. 2016: Pollen morphology of some Geranium subgenus Robertium species of Iran. Modern Phytomorphology 10: 39–45.

Knowles, L.L., & Carstens, B. 2007: Delimiting species without monophyletic gene trees. Systematic Biology 56: 887–895. doi:10.1080/10635150701701091.

Mayr, E. 1982: Te Growth of Biological Tought : Diversity, Evolution, and heritance. Cambridge, MA: Harvard University Press, 992 pp. Medrano, M., López-Perea E. & Herrera, C.M. 2014: Population genetics methods applied to a species delimitation problem: Endemic trumpet daffodils (Narcissus section Pseudonarcissi) from the Southern Iberian Peninsula. International Journal of Plant Sciences 175: 501–517. doi: 10.1086/675977

Meirmans, P.G. & Van Tienderen, P.H. 2004: GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Molecular Ecology Notes 4: 792–794.

Meirmans, P.G. 2012: AMOVA-based clustering of population genetic data. Journal of Heredity 103: 744–750.

Onsori, S., Salimpour, F. & Mazooji, A. 2010: Te new record of Geranium linearilobum Dc. based on anatomy and micromorphological study of pollen and seed, in Iran. Journal of plant environmental physiology 5: 21-30. [in Persian with English abstract]

Peakall, R. & Smouse, P.E. 2006: GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295.

Pérez-Losada, M., Eiroa, J., Mato, S., & Domínguez, J. 2005: Phylogenetic species delimitation of the earth worms Eiseniafetida (Savigny,1826) and Eiseniaandrei Bouché,1972 (Oligochaeta, Lumbricidae) based on mitochondrial and nuclear DNA sequences. Pedobiologia 49: 317–324.doi: 10.1016/j.pedobi.2005.02.004

Philipp, M. 1985: Reproductive biology of Geranium sessiliflorum, 1. Flower and flowering biology. New Zealand Journal of Botany 23: 567–589.

Podani, J. 2000: Introduction to the Exploration of Multivariate Data English translation. Backhuyes publisher, Leiden, 407 pp.

Pritchard, J.K., Stephens, M. & Donnelly, P. 2000: Inference of population structure using multilocus genotype Data. Genetics 155: 945–959.

Salimi Moghadam, N. 2015: Data from: Micromorphological studies on fruits and seeds of the genus Geranium (Geraniaceae) from Iran and their systematic signifcance – Dryad Digital Repository < http://dx.doi.org/10.5061/dryad. h1n71 >.

Salimpour, F., Mazooji, A. & Onsori, S. 2009: Stem and leaf anatomy of ten Geranium L. species in Iran. African Journal of Plant Science 3: 238–244.

Schluter, D. 2001: Ecology and the origin of species. Trends in Ecology & Evolution 16: 372–380.

Schönbeck-Temesy, E. 1970: Geraniaceae. In: Rechinger, K.H. ed., Flora Iranica, Vol. 69, pp. 30–58, Akademische Druck, Graz, Austria.

Sheidai, M., Zanganeh, S., Haji-Ramezanali, R., Nouroozi, M., Noormohammadi, Z. & Ghsemzadeh-Baraki, S. 2013: Genetic diversity and population structure in four Cirsium (Asteraceae) species. Biologia 68: 384–397.

Sites, J.W. & Marshall, J.C. 2003: Delimiting species: A Renaissance issue in systematic biology. Trends in Ecology & Evolution 18: 462–470.

Stebbins, G. L. 1957: Self fertilization and population variability in the higher plants. American Naturalist 91: 337–354.

Stebbins, G. L. 1970: Adaptive radiation of reproductive characteristics in angiosperms, I: pollination mechanisms. Annual Review of Ecology and Systematics 1: 307–326.

Weising, K., Nybom, H., Wolff, K. & Kahl, G. 2005: DNA Fingerprinting in Plants. Principles, Methods, and Applications. 2nd ed. CRC Press, Boca Rayton, 472 pp.

Wiens, J.J. & Penkrot, T.A. 2002: Delimiting species using DNA and morphological variation and discordant species limits in spinylizards (Sceloporus). Systematic Biology 51: 69–91.

Wiens, J.J. 2007: Species Delimitation: New approaches for discovering diversity. Systematic Biology 56: 875-878. doi:10.1080/10635150701748506.

Yeo, P. F. 1984: Fruit-discharge-type in Geranium (Geraniaceae): its use in classifcation and its evolutionary implications. Botanical Journal

Yeo, P. F. 2002: Hardy geraniums, ed. 2. Portland, Oregon: Timber Press, 218 pp.

Yeo, P. F. 2004: Te morphology and afnities of Geranium sections Lucida and Unguiculata. Te Linnean Society of London, Botanical Journal of the Linnean Society 144: 409–429.

Zohary, M. 1972: Flora Palaestina. Platanaceae to Umbelliferae. Vol. 4. Te Israel Academy of Sciences and Humanities, Jerusalem, Israel, 656 pp.

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