Population genetic structure and genetic diversity study in Lallemantia royleana (Benth.) Benth.: identification of potential gene pool in Iran
Keywords:
Gene flow, genetic fragmentation, ISSR, population assignment, Lallemantia.Abstract
Lallemantia royleana (Benth.) Benth. (Family Lamiaceae), is one of the most popular medicinal plants in Iran. It is an herbaceous pant that is commonly known as “Lady mantle”. The vernacular name of Lallemantia royleana's seed is Balangu or Balangu Shirazi that is used as a source of medicine. Medicinal plants are very important from economic point of view in Iran and several large industries are focused on medicinal plants cultivation, extraction and export. Therefore, providing data on the biology of these plants is important for the country. Lallemantia royleana grows in different parts of Iran and forms several local populations. Genetic, morphological and biochemical divergence of geographical populations are well known in plant species. We have no report on population genetic structure, genetic fragmentation, local adaptation and gen flow of Lallemantia royleana populations in the country. Therefore, the present population genetics investigation was programmed to produce data on above said questions. Randomly collected plants of 7 geographical regions were studied by ISSR molecular markers. AMOVA and Hickory test revealed significant genetic differentiation among these populations. They showed isolation by distance phenomenon and therefore, gene flow occurred between close-by located populations. STRUCTURE analysis identified two potential gene pools for Lallemantia royleana in IRAN. Some degree of gene flow and genetic admixture was identified by population assignment test. These information can be used in hybridization and gene conservation of this medicinal plant in Iran.
Downloads
References
Azizi,N., Sheidai,M., Mozafarian, V. & Noormohammadi, Z. 2014: Genetic, cytogenetic and morphological diversity in Helicrysum leucocephalum (Asteraceae) populations. Biologia 69:566-573
Çalişkan, M. 2012: Genetic Diversity in Plants Edited by, ISBN 978-953-51-0185-7, 510 pages, Publisher, In: Tech, Chapters published under CC BY 3.0 license.
Cao Shu, B. (Eds.) 1994: Lallemantia L., Flora of China, Lamiaceae. 17: 133-134.
Chen, X.Y. 2000: Effects of fragmentation on genetic structure of plant populations and implications for the biodiversity conservation. Acta Ecologica Sinica 20:884-892.
Di, X.Y., Liu K.W., Hou S.Q., Ji, P.L. & Wang, Y.L. 2014: Genetic variation of hazel (Carylusheterophylla) populations at different altitudes in Xingtagsi forest park in Huoshan, Shanxi, China. Plant omics jornal 7:213-220.
Ellis, J.R. & Burke, J.M. 2007: EST-SSRs as a resource for population genetic analyses. Heredity 99:125–132.
Freeland, J.R., Kirk, H. & Peterson, S.D. 2011: Molecular Ecology.In: Wiley-Blackwell, U.K. (2nded), 449.
Frichot, E., Schoville, S.D., Bouchard, G. & Francois, O. 2013: Testing for associations between loci and environmental gradients using latent factor mixed models. Molecular Biology and Evolution 30: 1687e1699.
Hamer, Ø., Harper, D.A.T. & Ryan, P.D. 2012: PAST: Paleontological Statistics software package for education and data analysis. Palaeontologia Electronica 4: 9.
Hedrick, P.W. 2005: A standardized genetic differentiation measure. Evolution 59:1633–1638.
Hou, Y., Lou, A. 2011: Population Genetic Diversity and Structure of a Naturally Isolated Plant Species, Rhodiola dumulosa (Crassulaceae). PLoS ONE6, e24497.
Huson, D.H. & Bryant, D. 2006: Application of Phylogenetic Networks in Evolutionary Studies. Molecular Biology and Evolution 23: 254-267.
Meirmans, P.G. 2012: AMOVA-based clustering of population genetic data. Journal Heredity 103:744-750.
Meirmans, P.G. &Van Tienderen, P.H. 2004: GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Molecular Ecology Notes 4: 792-794.
Minaeifar, A.A., Sheidai, M., Attar, F., Noormohammadi, Z. & Ghasemzadeh-Baraki B. 2015: Genetic and morphological diversity in Cousinia tabrisiana (Asteraceae) populations. Biologia 70: 328-338.
Murry, M.G. & Tompson, W.F.1980: Rapid isolation of high molecular weight plant DNA. Nucleic Acid Resear 8: 4321-4325.
Naghibi, F., Mosaddegh, M., Motamed, Ms. & Ghorban,i A. 2005: Labiatae family in folk medicine in Iran from ethnobotany to pharmacology. Iranian Journal of Pharmaceutical Research 2: 63-79.
Noormohammadi, Z., Shafaf, T., Farahani, F., Sheidai, M., Talebi, Sm. & Hasheminejad- Ahangarani- Farahani, Y. 2015: Within and among genetic variation in Asia flax Linum austriacum (Linaceae) in response to latitude change. Cytogenetic and molecular analyses 16:145-150.
Ohsawa, T. & Ide Y. 2008: Global patterns of genetic variation in plant species along vertical and horizontal gradients on mountains.Global Ecol.Biogeog 17:152-163.
Peakall, R. & Smouse, P.E. 2006: GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288-295.
Podani, J. 2000: Introduction to the Exploration of Multivariate Data [English translation], Leide, Netherlands, Backhuyes,
Setsuko, S., Ishida, K., Ueno, S., Tsu Mura, Y. &Tomaru, N. 2007: Population differentiation AND gene flow within a metapopulation of a threatened tree, Magnolia stellata (Magnoliaceae). American Journal of Botany 94: 128-136.
Sheidai, M., Zanganeh, S., Haji-Ramezanali, R., Nouroozi, M., Noormohammadi, Z. & Ghsemzadeh-Baraki, S. 2013: Genetic diversity and population structure in four Cirsium (Asteraceae) species. Biologia 68: 384-397.
Sheidai, M., Ziaee, S., Farahani, F., Talebi, S.M., Noormohammadi, Z. & Hasheminejad-Ahangarani Farahani, Y.2014: Infra-specific genetic and morphological diversity in Linum album (Linaceae). Biologia 69: 32e39.
Sheidai, M., Seif, E., Nouroozi, M. & Noormohammadi, Z. 2012: Cytogenetic and molecular diversity of Cirsium arvense (Asteraceae) populations in Iran. Journal of Japanese Botany 87: 193-205.
Slatkin, M. 1993: Isolation by distance in equilibrium and non-equilibrium populations. Evolution 47: 264-279.
Taberlet, P., Fumagalli, L. & Wust- Saucy, A.G. 1998: Comparative phylogegraphy and postgavial colonization routes in Europe. Molecular Ecology 7: 453-464.
Waples, R.S. 1998: Separating the wheat from the chaff patterns of genetic differentiation in high gene flow species .The American Genetic Association 89:438-450.
Weising, K., Nybom, H., Wolff, K. & Kahl, G. 2005: DNA Fingerprinting in Plants. In: Boca Rayton, F.l. (eds.), Principles, Methods, and ApplicationsCRC Press, USA, 4pp.72.
Downloads
Published
How to Cite
Issue
Section
License
Authors guarantee that the work is their own original creation and does not infringe any statutory or common-law copyright or any proprietary right of any third party. In case of claims by third parties, authors commit their self to defend the interests of the publisher, and shall cover any potential costs.
More in: Submission chapter
