Struktura mikrobne združbe v jami Dupnisa, Kırklareli, Turčija
DOI:
https://doi.org/10.3986/ac.v49i2-3.8575Ključne besede:
jamska mikrobiologija, raznovrstnost bakterij, raznovrstnost arhej, sekvenciranje naslednje generacijePovzetek
Stabilna temperatura, tema, velika zračna vlaga in omejena količina organske snovi so dejavniki, ki jamski ekosistem opredelijo kot ekstremno okolje in mu dajejo velik pomen v mikrobioloških študijah. Čeprav so bili izsledki študij z gojitvenimi metodami v jamah podobni izsledkom, pridobljenim z molekularnimi metodami, so zadnjenavedene omogočile določitev pestrejšega nabora mikroorganizmov. Spremenljivost mikroorganizmov se razlikuje glede na značilnosti posamezne jame. Cilj te študije je opredeliti raznovrstnost mikrobov v vzorcih iz petih predelov (vključno s turističnim predelom) jame Dupnisa in odkriti razlike med temi predeli. To je prva mikrobiološka študija jamskih sedimentov iz jamskega sistema Dupnisa na SZ Turčije. S sekvenciranjem Illumina Miseq naslednje generacije je bilo 14 debel in 298 rodov pripisanih bakterijskim OTU, 2 debli in 20 rodov pa arhejskim OTU. V bakterijski združbi prevladujejo debla Proteobacteria, Actinobacteria, Bacteroidetes, Gemmatimonadetes, Firmicutes, Nitrospirae, Chloroflexi in Acidobacteria, združbo arhej pa zastopata debli Thaumarchaeota in Euryarchaeota. Med bakterijami je vodilno deblo Proteobacteria, pri arhejah pa Thaumarchaeota. Indeks pestrosti Chao1 kaže na največjo pestrost bakterij na vzorčnem mestu AF (vhod v jamo/sediment), največjo pestrost arhej pa na vzorčnem mestu F2 (turistični del 2/jamski sediment). Glede na Shannon-Wienerjev indeks pestrosti je bila največja raznolikost rodov arhej in bakterij na vzorčnem mestu F2.
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