Antimicrobial resistant Escherichia coli from karst waters, surfaces and bat guano in Slovenian caves

  • Sara Skok
  • Blaž Kogovšek
  • Rok Tomazin
  • Samo Šturm
  • Jerneja Ambrožič Avguštin
  • Janez Mulec Karst Research Institute ZRC SAZU Titov trg 2 6230 Postojna
Keywords: caves, water, bats, guano, swabs, E. coli, antimicrobial resistance

Abstract

Escherichia coli, one of the primary intestinal commensal bacteria in humans and endothermic animals, is commonly considered an indicator of faecal pollution. E. coli strains were isolated from karst rivers under different hydrological conditions, from footpaths in tourist caves and from bat guano. Isolates were tested for phenotypic resistance to ampicillin, chloramphenicol, ciprofloxacin, nalidixic acid, tetracycline and trimethoprim. The highest percentage of antimicrobial resistant E. coli was found in karst waters, followed by those from surface swabs and from bat guano. Several isolates from rivers and swabs exhibited multidrug-resistant phenotype. Environmental conditions impact the populations of E. coli; a positive correlation between dissolved oxygen and E. coli counts, and a negative correlation between conductivity and E. coli concentrations have been observed for karst rivers. Malenščica (Slovenia), a drinking water resource with an extensive catchment area, contained a relative high percentage of antimicrobial-resistant E. coli strains. None of the isolates from bat guano was resistant to ampicillin, chloramphenicol, and tetracycline. Future monitoring of bats should consider a regular follow-up of indicative microbial disease indicators in fresh guano. Regular cleansing of tourist footpaths in caves and disinfection barriers at the cave entrances reduce the concentration and transmission of E. coli significantly. A future, more detailed, study on characterization of additional E. coli isolates is needed to reveal their pathogeneicity, mechanisms of antibiotic resistance, mobile genetic elements, and gene transfer frequencies to other members of the karst microbiome.

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Published
2020-12-16
How to Cite
1.
Skok S, Kogovšek B, Tomazin R, Šturm S, Ambrožič AvguštinJ, Mulec J. Antimicrobial resistant Escherichia coli from karst waters, surfaces and bat guano in Slovenian caves. AC [Internet]. 2020Dec.16 [cited 2021Dec.3];49(2-3). Available from: https://ojs.zrc-sazu.si/carsologica/article/view/9103
Section
Original papers