Molecular study on Senecio fontanicola (S. doria group, Asteraceae) and its conservation status

Avtorji

  • Elisa Pellegrini Department of Agriculture, Food, Environmental and Animal Science, University of Udine, via delle Scienze 206, 33100 Udine, Italy
  • Valentino Casolo Department of Agriculture, Food, Environmental and Animal Science, University of Udine, via delle Scienze 206, 33100 Udine, Italy
  • Duilio Iamonico Laboratory of Phytogeography and Applied Geobotany, Department of Planning, Design, and Technology of Architecture, University “La Sapienza”, Via Flaminia 72, 00196 Rome, Italy
  • Giuseppe Oriolo Via T. Ciconi 26, 33100 Udine, Italy
  • Nicola Rovere Via Mazzini, 14 - 33100 Udine, Italy
  • Massimo Vischi Department of Agriculture, Food, Environmental and Animal Science, University of Udine, via delle Scienze 206, 33100 Udine, Italy

Ključne besede:

alkaline fens, DNA barcoding, conservation status, Senecioneae

Povzetek

Senecio fontanicola is endemic to black-bog-rush fens of southern Austria, north-western Slovenia and north-eastern Italy. It is characterized by oblanceolate leaves, a low number of supplementary bracts and glabrous achenes and it grows in marshy spring areas, fens and reed beds, between elevations from 20 to 850 m . The species was never described with molecular traits and during the last decades, S. fontanicola showed a dramatic decline due to land reclamation for agriculture. Therefore, the present study aims to characterize S. fontanicola using the molecular barcoding technique and to updated its distribution to propose a global risk category for the species, based on IUCN criteria. The three molecular markers  used in this study (trnH-psbA, rbcL, and ITS) clearly distinguished S. fontanicola from S. doria. s.s.and the revised distribution allowed the definition of the conservation status of the species, that is Endangered-EN B2ab(i,ii,iii,iv) following the B criterion of the IUCN guidelines.

Prenosi

Podatki o prenosih še niso na voljo.

Literatura

Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. 1997: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25: 3389-3402.

Bartolucci, F., Peruzzi, L., Galasso, G., Albano, A., Alessandrini, A., Ardenghi, N. M. G., Astuti, G., Bacchetta, G., Ballelli, S., Banfi, E., Barberis, G., Bernardo, L., Bouvet, D., Bovio, M., Cecchi, L., Di Pietro, R., Domina, G., Fascetti, S., Fenu, G., Festi, F., Foggi, B., Gallo, L., Gottschlich, G., Gubellini, L., Iamonico, D., Iberite, M., Jiménez-Mejías, P., Lattanzi, E., Marchetti, D., Martinetto, E., Masin, R. R., Medagli, P., Passalacqua, N. G., Peccenini, S., Pennesi, R., Pierini, B., Poldini, L., Prosser, F., Raimondo, F. M., Roma-Marzio, F., Rosati, L., Santangelo, A., Scoppola, A., Scortegagna, S., Selvaggi, A., Selvi, F., Soldano, A., Stinca, A., Wagensommer, R. P., Wilhalm, T. & Conti, F. 2018: An updated checklist of the vascular flora native to Italy. Plant Biosystems 152(2): 179-303.

Bondesanf, M., Castiglioni, G. B., Elmis, C., Gabbianellis, G., Marocco, R., Pirazzolift, P. A. & Tomasin, A. 1995: Coastal areas at risk from storm surges and sea-level rise in northeastern Italy. Journal of Coastal Research 11(4): 1354-1379.

Bouckaert, R., Heled, J., Kühnert, D., Vaughan, T., Wu, C-H., Xie, D., Suchard, MA., Rambaut, A., & Drummond, A. J. 2014: BEAST 2: A Software Platform for Bayesian Evolutionary Analysis. PLoS Computational Biology 10(4): e1003537.

Calvo, J. & Aedo, C. 2015: A Taxonomic Revision of the Eurasian / Northwestern African Senecio doria Group (Compositae). Systematic Botany 40(3): 900-913.

Calvo, J., Álvarez, I., Aedo, C. & Pelser, P. B. 2013: A phylogenetic analysis and new delimitation of Senecio sect. Crociseris (Compositae: Senecioneae), with evidence of intergeneric hybridization. Taxon 62(1): 127-140.

Calvo, J., Vitek, E. & Aedo, C. 2014: (2295) Proposal to conserve the name Senecio doria (Compositae) with a conserved type. Taxon 63(3): 685-686.

CBOL Plant Working Group. 2009: A DNA barcode for land plants. PNAS, 106: 295-299.

Conti, F., Abbate, G., Alessandrini, A. & Blasi, C. 2005: An annotated Check-list of the Italian Vascular Flora. Roma: Palombi Editori, 428 pp.

Conti, F., Manzi, A. & Pedrotti, E. 1992: Il libro rosso delle piante d’Italia. W.W.F. and S.B.I. Camerino, 637 pp.

Conti, F., Manzi, A. & Pedrotti, E. 1997: Liste rosse regionali delle piante d'Italia. Dep.. of Botany and Ecology. University of Camerino, 139 pp.

Doyle, J. J. & Doyle, J. L. 1997: A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin 19: 11-15.

EEA. 2013: Available data for mapping and assessing ecosystems in Europe. Final Report-task 5.2.5_3_Ecosystem assessment: Identification of thematic datasets. Available: http://projects.eionet.europa.eu/eea-ecosystem-assessments/library/workingdocument-data-availability/ecosystem-assessment-data-availability_report_03-june-2013

Ehlers, J. & Gibbard, P. L. 2004: Quaternary Glaciations: Extent and Chronology Part I: Europe. Elsevier: London, 388 pp.

Ehrendorfer, F. & Hamman, U. 1965: Vorschläge zu einer floristischen Kartierung von Mitteleuropa. Berichte der Deutschen Botanischen Gesellschaft 78: 35-50.

Ewing, B. & Green, P. 1998: Basecalling of automated sequencer traces using Phred. II. Error probabilities. Genome Research 8: 186-194.

Fisher, M. A., Oswald, K. & Adler, W. 2008: Exkursionsflora für Österreich, Liechtenstein, und Südtirol. 3. Aufl.- Linz: Land Oberösterreich, Biologiezentrum der Oberösterr. Landesmuseen, 934 pp.

Fontana, A., Mozzi, P. & Marchetti, M. 2014: Alluvial fans and megafans along the southern side of the Alps. Sedimentary Geology 301: 150-171.

Frattini, S. 2008: Zone umide della pianura bresciana e degli anfiteatri morenici dei Laghi d’Iseo e di Garda (provincia di Brescia, regione Lombardia). Natura Bresciana 29: 93-95.

Gao, T., Yao, H., Song, J. Y., Liu, C., Zhu, Y. J., Ma, X. Y., Pang, X. H., Xu, H. X. & Chen, S. L. 2010: Identification of medicinal plants in the family Fabaceae using a potential DNA barcode ITS2. Journal of Ethnopharmacology 130: 116-121.

Grulich, V. & Hodálová, I. 1994: The Senecio doria Group (Asteraceae-Senecionae) in Central and Southeastern Europe. Phyton (Horn) Austria 34: 247-265.

Hartl, H., Kniely, G., Leute, G. H., Niklfeld, H. & Perko, M. 1992: Verbreitungsatlas der Farn- und Blütenpflanzen Kärntens. Klagenfurt, 325 pp.

Hollingsworth, P. M. 2008: DNA Barcoding plants in biodiversity hotspots: progress and outstanding questions. Heredity 101: 1-2.

Hollingsworth, M. L., Clark, A. A., Forrester, L. L., Richardson, J., Pennington, R. T., Long, D. G., Cowan, R., Chase, M. W., Gaudeul, M. & Hollingsworth, P. M. 2009: DNA Barcoding. Selecting Barcoding loci for plants: evaluation of seven candidate loci with species-level sampling in three divergent groups of land plants. Molecular Ecology Resources 9: 439-457.

Hollingsworth, P. M., Li, D. Z., van der Bank, M. & Twyford, A. D. 2016: Telling plant species apart with DNA: from barcodes to genomes. Philosophical Transactions of the Royal Society B. 371(1702): 20150338.

Iamonico, D. 2013: Lectotypification of Senecio doria (Asteraceae). Annales Botanici Fennici 50(1-2): 71-72.

Iamonico, D. 2017: Alien taxa of the tribe Senecioneae (Asteraceae) in Italy: a nomenclatural synopsis. Hacquetia 16(2): 281-292.

IUCN. 2012: IUCN Red List Categories and Criteria: Version 3.1. Second edition. Gland, Switzerland and Cambridge, UK: IUCN. iv + 32 pp.

Kang, Y., Deng, Z., Zang, R. & Long, W. 2017: DNA barcoding analysis and phylogenetic relationships of tree species in tropical cloud forests. Scientific reports 7(1):12564.

Khan, S., Al-Qurainy, F., Nadeem, M. & Tarroum, M. 2013: Selection of chloroplast DNA markers for the development of DNA barcode and reconstruction of phylogeny of Senecio asirensis boulos and jri wood. Pakistan Journal of Botany 45(2): 703-710.

Kimura, M. 1980: A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111-120.

Kress, W. J., Wurdack, K. J., Zimmer, E. A., Weigt, L. A. & Janzen, D. H. 2005: Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Science U.S.A. 102: 8369-8374.

Martini, F. & Poldini, L. 1986: Distribution and ecology of Erucastrum palustre (Pir.) Vis. Gortania, Atti Museo Friulano di Storia Naturale 8: 221-224.

Martini, F. & Poldini, L. 1987: Armeria helodes, a new species from North-Eastern Italy. Candollea 42(2): 533-544.

Martini, F. & Pavan, R. 2008: La flora vascolare spontanea di Pordenone: caratteri generali e distribuzione. Pordenone: Museo civico di storia natural, 277 pp.

Milton, J. J. 2009: Phylogenetic analyses and taxonomic studies of Senecioninae: Southern african Senecio section Senecio [thesis]. Saint Andrews: University of St Andrews, Scotland.

Niklfeld, H. & Schratt-Ehrendorfer, L. 1999: Rote Liste gefährdeter Farn- und Blütenpflanzen (Pteridophyta und Spermatophyta) Österreichs. 2. Fassung. In: Niklfeld, H. (eds.): Rote Listen gefährdeter Pflanzen Österreichs. Grüne Reihe des Bundesministeriums für Umwelt, Jugend und Familie 353-392.

Nordenstam, B. 2007: Tribe Senecioneae In: Kadereit, J.W. & Jeffrey, C. (eds.): Flowering Plants. Eudictos. Asterales. Vol. 8 of Kubitzki, J. (eds.): The Families and Genera of Vascular Plants. Springer, Berlin, Germany 208-241.

Pavan, R. & Costalonga, S. 2001: Flora delle zone umide dell'alto Livenza. Sacile: Associazione Naturalisti Sacile, 127 pp.

Pelser, P. B., Nordenstam, B., Kadereit, J. W. & Watson, L. E. 2007: An ITS phylogeny of Tribe Senecioneae (Asteraceae) and a new delimitation of Senecio L. Taxon 56: 1077-1104.

Pelser, P. B., Kennedy, A. H., Tepe, E. J., Shidler, J. B., Nordenstam, B., Kadereit, J. W. & Watson, L. E. 2010: Patterns and causes of incongruence between plastid and nuclear Senecioneae (Asteraceae) phylogenies. American Journal of Botany 97: 856-873.

Peruzzi, L., Bedini, G. & Andreucci, A. 2012: Homoploid hybrid speciation in Doronicum L. (Asteraceae)? Morphological, karyological and molecular evidences. Plant Biosystems 146(4): 867-877.

Pignatti, S., Menegoni, P. & Giacanelli, V. 2001: Liste rosse e blu della flora italiana. ANPA, 328 pp.

Pirona, J. A. 1855: Florae forojuliensis syllabus. Udine: Tip. Vendrame, 170 pp.

Poldini, L., Oriolo, G. & Vidali, M. 2001: Vascular Flora of Friuli-Venezia Giulia. An Annotated Catalogue and Synonimic Index. Studia Geobotanica 21: 3-227.

Poldini, L. & Oriolo, G. 2002: Alcune entità nuove e neglette per la flora italiana. Informatore Botanico Italiano 34: 105-114.

Rossi, G., Montagnani, C., Gargano, D., Peruzzi, L., Abeli, T., Ravera, S., Cogoni, A., Fenu, G., Magrini, S., Gennai, M., Foggi, B., Wagensommer, R. P., Venturella, G., Blasi, C., Raimondo, F. M. & Orsenigo, S. 2013: Lista Rossa della Flora Italiana 1. Policy Species e altre specie minacciate. Comitato Italiano IUCN e Ministero dell’Ambiente e della Tutela del Territorio e del Mare, 58 pp.

Sang, T., Crawford, D. J. & Stuessy, T. F. 1997: Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). American Journal of Botany 84: 1120-1136.

Scoppola, A. & Spampinato, G. 2005: Atlante delle specie a rischio di estinzione. CD-ROM In: Scoppola A, Blasi C. (eds.): Stato delle conoscenze sulla Flora vascolare Italiana. Roma: Palombi Editori, 253 pp.

Sheth, B. P.& Thaker, V. S. 2017: DNA barcoding and traditional taxonomy: an integrated approach for biodiversity conservation. Genome 60(7): 618-628.

Staden, R. 1996: The Staden Sequence Analysis Package. Molecular Biotechnology 5: 233-241.

Stanford, A. M., Hardenm R. & Parks, C. R. 2000: Phylogeny and biogeography of Juglans (Juglandaceae) based on matK and ITS sequence data. American Journal of Botany 87: 872-882.

Tamura, K. & Nei, M. 1993: Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10(3): 512-526.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. 2013: MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725-2729.

Thompson, J. D., Higgins, D. G. & Gibson, T. J. 1994: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673-4680.

Vreš, B., Seliškar, A. & Dakskobler, I. 2012: The phytosociological position of Senecio fontanicola Grulich & Hodálová, a rare and endangered species endemic to the Eastern Alps, in the successional sere on the montane wetland Zelenci (NW Slovenia). Wulfenia 19: 1-14.

Zenari, S. 1947: Intorno ad un Senecio delle Risorgive friulane. Istituto Botanico dell’Università di Padova, Lavori di Botanica. Pinerolo: G. Alzan, 1-5.

Prenosi

Objavljeno

2019-07-12

Kako citirati

Pellegrini, E., Casolo, V., Iamonico, D., Oriolo, G., Rovere, N., & Vischi, M. (2019). Molecular study on Senecio fontanicola (S. doria group, Asteraceae) and its conservation status. Hacquetia, 18(1). Pridobljeno od https://ojs.zrc-sazu.si/hacquetia/article/view/6802

Številka

Letn. 18 Št. 1 (2019)

Rubrike

Articles

Licenca

Avtorji jamčijo, da je delo njihova avtorska stvaritev, da v njem niso kršene avtorske pravice tretjih oseb ali kake druge pravice. V primeru zahtevkov tretjih oseb se avtorji zavezujejo, da bodo varovali interese založnika ter da bodo povrnili morebitno škodo.

Podrobneje v rubriki: Prispevki