Assessment of the conservation value of dry grassland habitats in the Inhulets River basin (Central Ukraine) based on vegetation and spider research

  • Nina Polchaninova V.N. Karazin Kharkiv National University, 4, Maidan Svobody, Kharkiv 61022, Ukraine
  • Olga Krasova Kryvyi Rih Botanical Garden of the National Academy of Sciences of Ukraine, 50, Marshaka Str., Kryvyi Rih 50089, Ukraine
  • Liudmyla Lysohor Kryvyi Rih State Pedagogical University, 54 Gagarin Av, Kryvyi Rih, 50086, Ukraine
  • Tatiana Atemasova V.N. Karazin Kharkiv National University, 4, Maidan Svobody, Kharkiv 61022, Ukraine
Keywords: conservation areas, protected plant species, spider fauna, steppe habitats, Ukraine


Dry grassland ecosystems are highly fragmented in Ukraine and increasingly threatened from intensification of farming practices. Evaluation of the habitat conservation importance based on multitaxon studies allows for optimizing selection of priority sites. We studied xerothermic habitats in the Kryvorizkyi Iron Ore Basin, an area of intensive mining and steel industry. Despite the high anthropogenic impact, the region has preserved areas of natural vegetation. Botanical research has been conducted since 2012, while spiders were investigated for the first time in 2017. In the four study sites, we recorded 265 vascular plant species and 95 spider species, including 23 protected plant species and 19 rare spider species. The studied habitats of forb-fescue-feather grass, petrophytic, meadow steppes, steppe scrub and siliceous rocks are under protection of the Bern Convention. Both vegetation and spider communities of the site Zelena Gully are well preserved and rich in rare and threatened species (18 plant and 11 spider species), which confirms its value as a priority site of the Emerald Network. Chervona Gully and the Slate Rocks should be included in the network. Frequent fires and intensive grazing in Khrystoforova Gully affect the spider diversity, decreasing the number of specialist and rare species, while early flowering plants benefit from this disturbance. Nevertheless, its vegetation is poorer than in Zelena and Chervona gullies.  Inclusion in the Emerald Network is critically important to the investigated sites, since expansion of mining activities is constantly threatening natural habitats in industrial regions. The control of burning, grazing and recreation will contribute to maintaining biodiversity in the protected areas.


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Anderson, S. 2002: Identifying important plant areas: a site selection manual for Europe. Plantlife International, 52 pp.

Batáry, P., Holzschuh, A., Orci, K.M., Samu, F. & Tscharntke, T. 2012: Responses of plant, insect and spider biodiversity to local and landscape scale management intensity in cereal crops and grasslands. Agriculture, Ecosystems and Environment 146 (1): 130–136.

Birkhofer K., Diekötter, T., Meub, C., Stötzel, K. & Wolters, V. 2015: Optimizing arthropod predator conservation in permanent grasslands by considering diversity components beyond species richness. Agriculture, Ecosystems and Environment 211: 65–72.

Braschler, B & Baur, B. 2016: Diverse effects of a seven-year experimental grassland fragmentation on major invertebrate groups. PLoS ONE 11(2): e0149567.

Buchar, J. & Růžička, V. 2002: Catalogue of spiders of the Czech Republic. Peres, Praha, 351 pp.

Buchholz, S. 2010: Ground spider assemblages as indicators for habitat structure in inland sand ecosystems. Biodiversity and Conservation 19(9): 2565–2595.

Cardoso, P., Silva, I., De Oliveira, N.G. & Serrano, A.R.M. 2004: Indicator taxa of spider (Araneae) diversity and their efficiency in conservation. Biological Conservation 120(4): 517–524.

Convention on the Conservation of European Wildlife and Natural Habitats 1979: Bern, 19.IX.1979 (ETS No 104).

Council Directive 92/43/EEC of 21 May 1992 on the conservation of natural habitats and of wild fauna and flora, O.J. L206, 22.07.92

Deli, O.F. 2014: Spider complexes of the ecosystems at the different transformation stages in the northwestern Black Sea Region. Abstract of thesis for the scientific degree of Candidate in Biological Sciences. I.I. Mechnikov Odessa National University, Odesa, 21 pp. [In Ukrainian with English summary].

Didukh, Y.P. (Ed.). 2009: Red Data Book of Ukraine. Plant Kingdom. Hlobalkonsaltynh, Kyiv, 912 pp. [In Ukrainian].

Didukh, Y.P. & Shelyag-Sosonko, Y.R. 2003: Geobotanical regioning of Ukraine and adjacent territories. Ukrainskyi Botanichnyi Zhurnal 60(1): 6–17 [in Ukrainian with English summary].

Dubyna, D.V., Dziuba, T.P., Yemelianova, S.M. et al. 2019: Prodrome of the vegetation of Ukraine. Naukovadumka, Kyiv. 783 pp. [In Ukrainian].

Gajdoš, P., Moscaliuc, L.A. & Rozwalka, R. (eds) 2014: Red list of spiders (Araneae) of the Carpathian Mts., pp. 82–135. In: Kadlečik, J. (ed.), Draft Carpathian red list of forest habitats and of species draft Carpathian list of invasive alien species, Štat na ochrana prirody Slovenskej republiky, Banska Bystrica, 234 pp.

Hammer, Ø., Harper, D.A.T. & Ryan, P.D. 2001: PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4 (1): 1–9.

Harlio, A., Kuussaari, M., Risto, K., Heikkinen, R.K. & Arponen, A. 2019: Incorporating landscape heterogeneity into multi-objective spatial planning improves biodiversity conservation of semi-natural grasslands. Journal for Nature Conservation 49: 37–44.

Iosypchuk, A.M. 2019: First data on the spider fauna (Araneae) of the National Nature Park ‘Kamyanska Sich’ (South Ukraine), In A.V. Akulov et al. (eds). From a Molecula to the Biosphere. Abstract of XІV International Young Scientists’ Conference (November 27th – 29th, 2019), Kharkiv, pp. 169–171.

IUCN Red List of Threatened Species. Version 2020-1. International Union for Conservation of Nature and Natural Resources. Mode of access (Accessed on 15 October 2020).

Knappová, L., Hemrova, L. & Münzbergová, Z. 2012: Colonization of central European abandoned fields by dry grassland species depends on the species richness of the source habitats: a new approach for measuring habitat isolation. Landscape Ecology 27: 97–108.

Kontar, I.S. 2001: Differentiation of the Polissya and Forest Steppe of Ukraine crystal rock outcrops vegetation cover. Abstract of thesis for the scientific degree of Candidate in Biological Sciences. M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Kyiv, 20 pp. [in Ukrainian with English summary].

Kovblyuk, M.M. & Kastrygina, Z.А. 2015: Updated catalogue of the spiders (Arachnida, Aranei) of the Crimea. Ukrainska Entomofaunistyka 6(2): 1–81 [in Russian].

Krasova O.O., Polchaninova N.Yu. & Lysogor L.P. 2020: Xerothermic habitats of the Kryvyi Rih part of the River Inhulets basin: conservation value in botanical and arachnological aspects. In: Monitoring and biodiversity conservation in Ukraine: Plants and fungi / Series: ‘Conservation Biology in Ukraine’ 16(1), Druk Art, Kyiv, Chernivtsi, pp. 116–125. [in Ukrainian].

Kuzemko, A.A., Didukh, Ia. P., Onyshchenko, V.A. & Sheffer, Ya. (eds) 2018: National habitat catalogue of Ukraine. FOP Klymenko Iu.Ia., Kyiv, 442 pp. [In Ukrainian].

Lafage, D., Maugenest, S., Bouzillé, J.-B. & Pétillon, J. 2015: Disentangling the influence of local and landscape factors on alpha and beta diversities: opposite response of plants and ground plants ground-dwelling arthropods in wet meadows. Ecological Research 30: 1025–1035.

Magurran, A. 1991: Ecological diversity and its measurements. Chapman and Hall, London, 178 pp.

Mikhailov, K.G. 2013: The spiders (Arachnida: Aranei) of Russia and adjacent countries: a non-annotated checklist. Arthropoda Selecta, Supplement 3: 1–262.

Nentwig, W., Blick, T., Gloor, D., Hänggi, A. & Kropf, C. 2020: Araneae. Version 12.2020. (accessed 6.12.2020)

Niemelä, R. & Baur, B. 1998: Threatened species in a vanishing habitat: plants and invertebrates in calcareous grasslands in the Swiss Jura Mountains. Biological Conservation 7: 1407–1416.

Öberg, S., Mayr, S. & Dauber, J. 2008: Landscape effects on recolonisation patterns of spiders in arable fields. Agriculture, Ecosystems and Environment 123: 211–218.

Onyshchenko, V.A. (ed.) 2017: Important Plant Areas of Ukraine. Alterpress, Kyiv, 376 pp.

Outhwaite, C.L., Gregory, R.D., Chandler, R.E., Collen, B. & Isaac, N.J.B. 2020: Complex long-term biodiversity change among invertebrates, bryophytes and lichens. Nature Ecology and Evolution 4: 384–392.

Perner, J. & Malt, S. 2003: Assessment of changing agricultural land use: Response of vegetation, ground-dwelling spiders and beetles to the conversion of arable land into grassland. Agriculture, Ecosystems and Environment 98: 169–181.

Poggio, S.L., Chaneton, E.J. & Ghersa, C.M. 2010: Landscape complexity differentially affects alpha, beta, and gamma diversities of plants occurring in fencerows and crop fields. Biological Conservation 143: 2477–2486.

Polchaninova, N. 2012: Assemblages of herb-dwelling spiders (Araneae) of various steppe types in Ukraine and the Central Chernozem region of Russia. ArachnologisheMitteilungen Bd. 43 (2): 66–78.

Polchaninova, N.Yu., Gnelitsa, V.A., Evtushenko, K.V. & Singaevsky, E.N. 2017: An annotated checklist of spiders (Arachnida: Aranei) of the National Nature Park ‘Buzkyi Hard’ (Mykolaiv Area, Ukraine). Arthropoda Selecta 26(3): 253–272.

Polchaninova, N.Yu. &Prokopenko, E.V. 2013: Catalogue of the spiders (Arachnida, Aranei) of Left-Bank Ukraine. Arthropoda Selecta, Supplement 2: pp. 1–268.

Polchaninova, N.Yu. &Prokopenko, E.V. 2017: Catalogue of the spiders (Arachnida, Aranei) of Left-Bank Ukraine. Addendum I. 2013–2016. Mikhailov K.G. (ed.). Arthropoda Selecta, Supplement 4: pp. 1–115.

Polchaninova, N. & Prokopenko, E. 2019: An updated checklist of spiders (Arachnida: Araneae) of Left-Bank Ukraine. Arachnologische Mitteilungen 57(1): 60–64.

Polchaninova, N., Savchenko, G., Ronkin, V., Drogvalenko, A. & Putchkov, A. 2019: Summer fire in steppe habitats: a long-term effect on vegetation and autumnal assemblages of cursorial arthropods. Hacquetia 18: 213–231.

Ponomarev, A.V. 2017: Spiders (Arachnida: Aranei) of steppe and meadow-steppe habitats of gully and ravine ecosystems of the valley of the Don River lower reaches. Proceedings of the Russian Entomological Society 88 (1): 118–131 [in Russian with English summary].

Pott, R. 2011: Phytosociology: A modern geobotanical method. Plant Biosystems 145: 9–18.

Prokopenko, E.V. & Zhukov, O.V. 2018: Spider (Aranei) steppe community in a ravine with fescue–feather-grass petrophytic vegetation. Acta Biologica Sibirica 4: 17–21. [in Russian with English summary]

Revised Annex I of Resolution 4 (1996) of the Bern Convention on endangered natural habitats types using the EUNIS habitat classification (Adopted by the Standing Committee on 6 December 2019). Mode of access

Řezáč, M., Kůrka, A., Růžička, V. & Heneberg, P. 2015: Red list of Czech spiders: 3rd edition, adjusted according to evidence-based national conservation priorities. Biologia 70: 645–666.

Rodriguez-Artigas, S.M., Ballester, R. & Corronca, J.A. 2016: Factors that influence the beta-diversity of spider communities in northwestern Argentine an Grasslands. PeerJ, pp. 1–19.

Smetana, О.М. & Smetana, N.М. 2005: Structure of terrestrial mesofauna in Kryvbas. Phitosotsiotsentr, Kyiv, 227 pp. [in Russian].

Sobko, V.G. 1972: Endemic and relic elements of the flora of the granite outcrops of the Dnieper Upland. Ukrainian Botanical Journal 29 (5): 624–630. [in Ukrainian]

Szmatona-Túri, T., Vona-Túri, D., Urbán, L., Weiperth, A. & Magos, G. 2019: How grassland management methods affect spider diversity. Acta–Universitatis–Sapientiae, Agriculture and Environment 11: 23–37.

Tamme, R., Hiiesalu, I., Laanisto, L., Szava-Kovats, R. & Pärtel, M. 2010: Environmental heterogeneity, species diversity and co-existence at different spatial scales. Journal of Vegetation Science 21(4): 796–801.

Tokarsky, V.A. (ed.) 2013: Red Data Book of Kharkiv Region of Ukraine. Animals. V. N. Karazin Kharkiv National University, Kharkiv, 472 pp. [in Ukrainian]

Updated list of officially adopted Emerald sites (December 2019). Mode of access

WSC 2020: World Spider Catalog. Natural History Museum Bern, online at, version 21.5

Zalevskiy, V.D. & Bronskov, A.I. (eds.) 2017: Red Data Book of Donetsk Region: Animals. Scientific information guide. PJSC “Vinnytsia Regional Printing House»”, Vinnytsia, 452 pp. [in Ukrainian].

Zao, Z., Reddi, G.V.P., Wei, S., Zhu, M., Zhang, K., Yu, H., Wang Z., Jiang, Q. & Zhang, R. 2018: Plant cover associated with aboveground net primary productivity (ANPP) mediates insect community composition in steppes of Northwest China. Journal of Asia-Pacific Entomology 21: 361–366.

Zografou, K., Adamidis, G.C., Komnenov, M., Kati, V., Sotirakopoulos, P., Pitta, E. & Chatzaki, M. 2017: Diversity of spiders and orthopterans respond to intra-seasonal and spatial environmental changes. Journal of Insect Conservation 21(3): 531–543.
How to Cite
PolchaninovaN., KrasovaO., LysohorL. and AtemasovaT. (2020) “Assessment of the conservation value of dry grassland habitats in the Inhulets River basin (Central Ukraine) based on vegetation and spider research”, Hacquetia, 20(1), pp. 225–242. Available at: (Accessed: 8May2021).