Pollen morphological study of some species of the genus Rubus L. (Rosaceae) of the flora of Armenia
Keywords:
brambles, pollen aperture, exine ornamentation, light microscopy, scanning electron microscopyAbstract
Pollen morphology of 10 Armenian species of the genus Rubus L. was studied using both light microscopy (LM) and scanning electron microscopy (SEM), including the species R. candicans, R. cartalinicus and R. takhtadjanii investigated for the first time. The data received revealed the significant palynomorphological uniformity in the aperture type (mainly 3-zonocolp-orate, geniculate), occasionally 4-zonocolp-orate one. In some 4-zonocolp-orate pollen grains of the species R. armeniacus, additional slit-like or colpus-like areas were also noted at one or both poles. Exine ornamentation was predominantly finely striate-perforate (SEM), sometimes in combination with finely striate-microreticulate one (R. armeniacus and R. caesius). Pollen grains were small, sometimes of medium size also. The smallest pollen grains (according to polar axis length) were observed in the species R. takhtadjanii (on average 14,2 μm), and the largest ones in R. caucasicus (on average 26.3 μm). Our data support the previously held view that pollen grain morphology within the genus Rubus can only be used as an auxiliary feature for the diagnosis of individual species.
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References
Alice, L. A. (2002). Evolutionary relationships in Rubus (Rosaceae) based on molecular data. Acta Horticulturae, 585, 79–83. https://doi.org/10.17660/ActaHortic.2002.585.9
Alice, L. A. , & Campbell, C. S. (1999). Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. American Journal of Botany, 86, 81–97. DOI:10.2307/2656957
Angiosperm Phylogeny Website, https://www.mobot.org/mobot/research/apweb/
Avetisyan, E. M. (1950). Uproshchenny atsetolizny metod obrabotki pyl’tsi [Simplified acetolyzed method of the pollen grains treatment]. Botanicheskii Zhurnal, 35(4), 385–387 (in Russian).
Avetisyan, E. M. & Manukyan, L. K. (1958). Opisanie pyl’tsevykh zeren semeystv Buxaceae, Grossulariaceae, Platanaceae, Rosaceae [Description of the pollen of Buxaceae, Grossulariaceae, Platanaceae, Rosaceae]. In Takhtajan, A. L. (Ed.). Flora Armenii [Flora of Armenia], 3 (p. 37). Academy of Sciences of Armenia, Yerevan (in Russian).
Carter, K. A., Liston, A., Bassil, N. V., Alice, L. A., Bushakra, J. M., Sutherland, B. L., Mockler, T. C., Bryant, D. W., & Hummer, K. E. (2019).Target capture sequencing unravels Rubus evolution. Frontiers in Plant Science, 10, 1615. https://doi.org/10.3389/fpls.2019.01615
Chen, R. Y., Song, W. Q., Liang, G. L., Lin, S. H., Li, X. L., & An, Z. P. (1993). Chromosome atlas of Chinese principal economic plants. Volume 1. Chromosome atlas of Chinese fruit trees and their close wild relatives. International Academic Publishers, Beijing, China.
Chen, Z.-D., Yang, T., Lin, L., Lu, L.-M., Li, H.-L., Sun, M., Liu,B., Chen, M., Niu, Y.-T., Ye, J.-F., Cao,Zh.-Y., Liu, H.-M., Wang, X.-M., Wang, W., Zhang, J.-B., Meng, Zh., Cao,W., Li, J.-H., Wu, Sh.-D., Zhao H.-L., Liu, Zh.-J., Du, Zh.-Y., Wang, Q.-F., Guo, J., Tan, X.-X., Su, J.-X., Zhang, L.-J., Yang, L.-L., Liao, Y.-Y., Li, M.-H., Zhang, G.-Q., Chung, Sh.-W., Zhang, J., Xiang, K.-L., Li, R.-Q., Soltis, D. E., Soltis, P. S., Zhou, Sh.-L., Ran, J.-H., Wang, X.-Q., Jin, X.-H., Chen, Y.-Sh., Gao, T.-G., Li, J.-H., Zhang, Sh.-Zh., & Lu, A.-M. (2016). China Phylogeny Consortium. Tree of life for the genera of Chinese vascular plants. Journal of Systematics and Evolution, 54(4), 277–306. https://doi.org/10.1111/jse.12219
Chen, X., Li, J., Cheng, T., Zhang, W., Liu, Y., Wu, P., Yang, X., Wang, L., & Zhou, Sh. (2020). Molecular systematics of Rosoideae (Rosaceae). Plant Systematics and Evolution, 9, 306. https://doi.org/10.1007/s00606-020-01629-z
Connolly, T. J. (1999). Newberry Crater: A ten-thousand-year record of human occupation and environmental change in the basin-plateau borderlands. Anthropological Papers, 121, University of Utah, Salt Lake City, UT.
Daehler, C. C. (1998). The taxonomic distribution of invasive angiosperm plants: Ecological insights and comparison to agricultural weeds. Biological Conservation, 84, 167–180. https://doi.org/10.1016/S0006-3207(97)00096-7
Demchenko, N. I. (1967). O pyl’tse Rubus chamaemorus [On the pollen of Rubus chamaemorus]. Botanicheskii Zhurnal, 52(4), 535–538 (in Russian).
Eide, F. (1981a). On the pollen morphology of Rubus chamaemorus L. (Rosaceae). Grana, 20(1), 25–27. https://doi.org/10.1080/00173138109436733
Eide, F. (1981b). Key for Northwest European Rosaceae pollen. Grana, 20(2), 101–118. https://doi.org/10.1080/00173138109427651
Erdtman, G. (1952). Pollen morphology and plant taxonomy 1. Angiosperms. Almqvist et Wiksell, Stockholm.
Faegri, K., & Iversen, J. (1989). Textbook of pollen analysis (Fourth edition). John Wiley & Sons, Chichester.
Fayvush, G. M., & Aleksanyan, A. S. (2016). Habitats of Armenia. NAS RA, Institute of Botany, Yerevan.
Focke, W.O. (1910). Species Ruborum. Monographiae generis Rubi prodromus I. Bibliotheca Botanica, 17(72), 1–120. http://dx.doi.org/10.5962/bhl.title.15533
Focke, W.O. (1911). Species Ruborum. Monographiae generis Rubi prodromus II. Bibliotheca Botanica, 17(72), 121–223. http://dx.doi.org/10.5962/bhl.title.15533
Focke, W.O. (1914). Species Ruborum. Monographiae generis Rubi prodromus III. Bibliotheca Botanica, 19(83), 1–274. http://dx.doi.org/10.5962/bhl.title.15533
Foster, T. M., Bassil, N. V., Dossett, M., Worthington, M. L., & Graham, J. (2019). Genetic and genomic resources for Rubus breeding: a roadmap for the future. Horticulture Research, 6, 116. https://doi.org/10.1038/s41438-019-0199-2
Gao, X.-F., Xiong, X.-H., Boufford, D. E., Gao, Y.-D., Xu, B., & Zhang, C. (2023). Phylogeny of the diploid species of Rubus (Rosaceae). Genes, 14(6), 1152. https://doi.org/10.3390/genes14061152
Ghosh, A., & Saha, I. (2017). Pollen morphological study of some selected Indian taxa of Rosaceae. Indian Journal of Applied and Pure Biology, 32(2), 121–130.
Gonzalez Romano, M. L., & Candau, P. A. (1989). Contribution to palynological studies in the Rosaceae. Acta Botánica Malacitiana, 14: 105–116.
Gustafsson, A. (1943). The genesis of the European blackberry flora. Acta Universitatis Lundensis, 36(6), 1–200.
Halbritter, H., Ulrich, S., Grimsson, F., Weber, M., Zetter, R., Hesse, M., Buchner, R., Svojtka M., & Frosch-Radivo, A. (2018). Illustrated Pollen Terminology (2-nd Edition). Springer, Wien. https://doi.org/10.1007/978-3-319-71365-6
Hanchana, K., Saensouk, S., & Saensouk, P. (2023). Pollen morphology and anatomy of Rubus L. (Rosaceae) in Thailand. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(1), Article number 13070. https://doi.org/10.15835/nbha51113070
Hayrapetyan, A., & Bruch A. A. (2020). Pollen morphology of some species of the genus Quercus L. (Fagaceae) in the Southern Caucasus and adjacent areas. Acta Palaeobotanica, 60(1): 1-42. https://doi.org/10.35535/acpa-2020-0001
Hayrapetyan, A. M., Fayvush, G. M., & Muradyan, A. G. (2017). Predstavlennost’ redkikh endemichnykh vidov rasteniy Armenii v gerbarii Instituta botaniki NAN RA (ERE). Chast’ V. Informatsiya po otdel’nym vidam (semeystva Poaceae – Rosaceae) [Representativeness of rare endemic plant species in the herbarium of the Institute of botany NAS RA (ERE). Part V. Information on some species (fam. Poaceae – Rosaceae)]. Biological Journal of Armenia, 3(69), 12–17 (in Russian).
Hebda, R. J., & Chinnappa, C. C. (1990). Studies on pollen morphology of Rosaceae in Canada. Review of Palaeobotany and Palynology, 64, 103–108. https://doi.org/10.1016/0034-6667(90)90123-Z
Hebda, R. J., & Chinnappa, C. C. (1994). Studies on pollen morphology of Rosaceae. Acta Botanica Gallica, 141(2), 183–193. https://doi.org/10.1080/12538078.1994.10515150
Hendrickson, J. (1981). The berry book. Doubleday, New York, NY.
Huang, T.-R., Chen, J.-H., Hummer, K. E., Alice, L. A., Wang, W.-H., He, Y., Yu, S.-X., Yang, M.-F., Chai, T.-Y., Zhu, X.-Y., Ma, L.-Q., & Wang, H. (2023). Phylogeny of Rubus (Rosaceae): Integrating molecular and morphological evidence into an infrageneric revision. Taxon, 72(2), 278–306. https://doi.org/10.1002/tax.12885
Hummer, K. E. (2010). Rubus pharmacology: Antiquity to the present. Hortscience, 45(11), 1587–1591. https://doi.org/10.21273/HORTSCI.45.11.1587
Ikuse, M. (1955). General survey list of pollen grains in Japan (7). Rosales. 4. Journal of Japanese Botany, 30, 365–368.
Ivanova, D. (2005). Mediterranean chromosome number reports 15 (1445–1456). Flora Mediterranea, 15, 719–728.
Kosenko, V. N., Hiep N. T., & Yakovlev, G. P. (1984). Palinomorfologicheskoe izuchenie predstaviteley roda Rubus (Rosaceae) flory V’etnama [Palynomorphological study of the representatives of the genus Rubus (Rosaceae) in the flora of Viet-Nam]. Botanicheskii Zhurnal, 69(4), 497–503 (in Russian).
Krahulcová, A., & Holub, J. (1997). Chromosome number variation in the genus Rubus in the Czech Republic. II. Preslia 69, 289–310.
Kuprianova, L. A. (1978). Genus Rubus L. In Kuprianova, L. A., & Alyoshina, L. A. (Eds.). Pyl’tsa dvudol’nykh rasteniy flory Yevropeyskoy chasti SSSR [Pollen and spores of plants from the flora of European part of the USSR]. 2. Lamiaceae-Zygophyllaceae. Nauka, Leningrad (in Russian).
Kuprianova, L. A., & Alyoshina, L. A. (1967). Palinologicheskaya terminologiya pokritosemennikh rasteny [Palynological terminology of angiosperms]. Nauka, Leningrad (in Russian).
Kurtto, A., Weber, H. E., Lampinen, R., & Sennikov, A. N. (Eds.) (2011). Atlas florae Europaeae. Distribution of vascular plants in Europe. 15: Rosaceae (Rubus). The Committee for Mapping the Flora of Europe and Societas Biologica Fennica Vanamo, Helsinki. DOI:10.2307/41549119
Lechowicz, K․, Wrońska-Pilarek, D․, Bocianowski, J․, & Maliński, T․ (2020) Pollen morphology of Polish species from the genus Rubus L. (Rosaceae) and its systematic importance. PLoS ONE 15(5): e0221607. https://doi.org/10.1371/journal.pone.0221607
Lechowicz, K., Bocianowski, J., & Wrońska-Pilarek, D. (2021). Pollen morphology and variability of species from the genus Rubus L. (Rosaceae) alien and invasive in Poland. Webbia. Journal of Plant Taxonomy and Geography, 76(1), 109–121. https://doi.org/10.36253/jopt-10355
Lechowicz, K., Bocianowski, J., & Wrońska-Pilarek, D. (2022). Pollen morphological inter- and intraspecific variability in selected species of Rubus L. (Rosaceae). Forests 13, 1946. https://doi.org/10.3390/f13111946
Li, W.-L., He, S.-A., Gu, Y., Shu, P., & Pu, Z.-M. (2001). Pollen morphology of the genus Rubus from China. Acta Phytotaxonomica Sinica, 39(3), 234–247.
Lu, L.-T. (1983). A study on the genus Rubus of China. Acta Phytotaxonomica Sinica, 21(1), 13–25.
Mabberley, D. J. (2017). Mabberley’s plant-book: A portable dictionary of plants, their classification and uses (4th edition). Cambridge University Press, Cambridge. https://doi.org/10.1017/9781316335581
Monasterio-Huelin, E., & Pardo, C. (1995). Pollen morphology and wall stratification in Rubus L. (Rosaceae) in the Iberian Peninsula. Grana, 34, 229–236. https://doi.org/10.1080/00173139509429050
Mulkijanyan, Ya. I. (1958). Genus Rubus. In Takhtajan, A. L. (Ed.), Flora Armenii [Flora of Armenia], 3 (pp. 37-66). Academy of Sciences of Armenia, Yerevan (in Russian).
Nybom, H. (1986). Chromosome numbers and reproduction in Rubus subgen. Malachobatus. Plant Systematics and Evolution, 152(3/4), 211–218.
Potonié, R. (1934). I. Zur Morphologie der fossilen Pollen und Sporen. Arbeiten aus dem Institut für Paläobotanik und Petrographie der Brennsteine, 4, 5–24.
Punt, W., Hoen, P. P., Blackmore, S., Nilsson, S., & Le Thomas, A. (2007). Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology, 143(1−2), 1−81. https://doi.org/10.1016/j.revpalbo.2006.06.008
Reitsma, Tj. (1966). Pollen morphology of some European Rosaceae. Acta Botanica Neerlandica, 15(2), 290−307. https://doi.org/10.1111/j.1438-8677.1966.tb00234.x
Runemark, H. (2006). Mediterranean chromosome number reports 16 (1473–1571). Flora Mediterranea, 16, 408–425.
Smolyaninova, L. A., & Golubkova, V. F. (1950). K metodike issledovaniya pyl’tsi [To pollen research method]. Doklady Akademii Nauk SSSR, 75(1), 125−126 (in Russian).
Sochor, M., Vasut, R. J., Sharbel, T. F., & Travnicek, B. (2015). How just a few makes a lot: Speciation via reticulation and apomixes on example of European brambles (Rubus subgen. Rubus, Rosaceae). Molecular Phylogenetics and Evolution, 89, 13–27. https://doi.org/10.1016/j.ympev.2015.04.007
Surova, T. G. (1975). Elektronno-mikroskopicheskoe issledovanie pyl’tsy i spor rasteniy [Electron microscope investigation of plants pollen and spores]. Nauka, Moscow (in Russian).
Takhtajan, A. (1997). Diversity and classification of flowering plants. Columbia University Press, New York.
Takhtajan, A. (2009). Flowering Plants (Second Edition). Springer Science+Business Media B.V. https://doi.org/10.1007/978-1-4020-9609-9
Tamanyan, K., Fayvush, G., Nanagyulyan, S., & Danielyan, T. (eds.) (2010). The Red Book of Plants of the Republic of Armenia. Higher Plants and Fungi (Second edition). Zangak, Yerevan.
The Plant List. Genus Rubus. http://www.theplantlist.org/tpl1.1/search?q=Rubus
Thiem, B. (2003). Rubus chamaemorus L. – a boreal plant rich in biologically active metabolites: A review. Biology Letters, 40, 3–13.
Tomlik-Wyremblewska, A. (1995). Pollen morphology of genus Rubus L. Part I. Introductory studies of the European representatives of the subgenus Rubus L. Acta Societatis Botanicorum Poloniae, 64, 187–203.
Tomlik-Wyremblewska, A. (2000). Pollen morphology of genus Rubus L. Part II. Introductory studies on the Malesian species of subgenus Micranthobatus Fritsch. Acta Societatis Botanicorum Poloniae, 69, 31–40.
Tomlik-Wyremblewska, A., Van Der Ham, R. W. J. M., & Kosiński, P. (2004). Pollen morphology of genus Rubus L. Part III. Studies on the Malesian species of subgenera Chamaebatus L. and Idaeobatus L. Acta Societatis Botanicorum Poloniae, 73, 207–227.
Ueda, Y. (1992). Pollen surface morphology in the genus Rosa and related genera. Japanese Journal of Palynology, 38, 94–105.
Valdés, B., Díez, M. J., & Fernández, I. (1987) Atlas polínico de Andalucía occidental. Universidad de Sevilla, Sevilla.
Van der Pluym, A., & Hideux, M. (1997). Applications d’une methodologie quantitative a la palynologie d’Eryngium maritimum (Umbelliferae). Plant Systematic and Evolution, 127, 55–85. http://dx.doi.org/10.1007/BF00984142
Wronska-Pilarek, D., Maliński, T., & Lira, J. (2006). Pollen morphology of Polish species of genus Rubus – Part I. Rubus gracilis. Dendrobiology, 56, 69–77.
Wronska-Pilarek, D., Jagodzinski, A. M., & Malinski, T. (2012). Morphological studies of pollen grains of the Polish endemic species of the genus Rubus (Rosaceae). Biologia (Section Botany), 67(1), 87–96. https://doi.org/10.2478/s11756-011-0141-z
Xiong, X.-H., Zhou, X.-M., Li, M., Xu, B., Deng, H.-N., Yu, Q., & Gao, X.-F. (2019). Pollen morphology in Rubus (Rosaceae) and its taxonomic implications. Plant Systematics and Evolution, 305, 705–716. https://doi.org/10.1007/s00606-019-01600-7
Zhou, L.-H., Wei, Z.-X., & Wu, Z.-Y. (1999). Pollen morphology of Rosoideae (Rosaceae) of China. Acta Botanica Yunnanica, 21, 455–460.
Zieliński, J. (2004). The genus Rubus (Rosaceae) in Poland. Polish Botanical Studies, 16, 1–300.
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