Genetic diversity and morphological variability of Iranian Silene aucheriana populations inferred from nrDNA ITS sequences and morphological analysis

Authors

Keywords:

morphological variation, nrDNA ITS, quantitative traits, Silene aucheriana

Abstract

We conducted a comprehensive study on the diversity of Silene aucheriana Boiss. populations by analyzing both morphological data and nrDNA ITS sequences. Maximum parsimony and Bayesian methods on representative material from 15 populations throughout Iran demonstrated that they did not form a monophyletic group. Strong positive correlation occurred between epipetalous filaments length to claw (EFLC), basal leaf length (BLL), basal leaf width (BLW), and plant height (PLH) with elevation. While negative correlation achieved between anthophore length (AnL) with the average air temperature and rainfall. Canonical Correspondence Analysis (CCA) showed that most morphological traits were more correlated to elevation than to average rainfall and temperature. Multivariate analysis of morphological traits with ITS analysis displayed a slight divergence between two types of regions based on their elevation.

Downloads

Download data is not yet available.

References

Brothers, A., Weingartner, L., & Delph, L. (2016). Genetically based population divergence of Silene latifolia from two climate regions. Evolutionary ecology research, 17, 637–650.

Chowdhuri, P. K. (1957). Studies in the genus Silene. Note from the Royal Botanic Garden, Edinburgh, 22, 221–278

Coode, M. J., & Cullen, J. (1967). Silene L. In: Davis, P.H. (Ed.). Flora of Turkey and the East Aegean Islands Edinburgh University Press pp. 179-242.

Del Valle, J.C., Soriguer, I., Buide, M., Narbona, E., & Whittall, J.B. (2019). Whole plastome sequencing within Silene section Psammophilae reveals mainland hybridization and divergence with the Balearic Island populations. Frontiers in Plant Science, 10, 1466. https://doi.org/10.3389/fpls.2019.01466

Douzery, E. P., Pridgeon, A. M., Kores, P. Linder, H. P., Kurzwell, H., & Chase, M. (1999). Molecular phylogenetics of Diseae (Orchidaceae) a contribution from nuclear ribosomal ITS sequences. American Journal of Botany, 86(6), 887–899. https://doi.org/10.2307/2656709

Doyle, J., & Doyle, L. (1990). Isolation of Plant DNA from Fresh Tissue. Focus, Vol 12, No. 1, pp. 13-15

Duminil, J. Di., & Michele, M. (2009). Plant species delimitation a comparison of morphological and molecular markers. Plant Biosystems, 143, 528–542. https://doi.org/10.1080/11263500902722964

Durović, S., Schönswetter, P., Niketić, M., Tomović, G., & Frajman, B. (2017). Disentangling relationships among the members of the Silene saxifraga alliance (Caryophyllaceae) Phylogenetic structure is geographically rather than axonomically segregated. Taxon, 66, 343– 364. https://doi.org/10.12705/662.4

Fick, S. E., & Hijmans, R. J. (2017). WorldClim 2: New 1‐km spatial resolution climate surfaces for global land areas: New climate surfaces for global land areas. The International Journal of Climatology, 37(12), 4302–4315. https://doi.org/10.1002/joc.5086

Firat, M., & Yıldız, K. (2016). Silene konuralpii (Sect. Spergulifoliae, Caryophyllaceae), a new species from eastern Anatolia. Phytotaxa, 288, 214–226.

Ghazanfar, S. A. (1984). Pollen morphology of the genus Silene L. (Caryophyllaceae) sections Siphonomorpha otth and Auriculatae (boiss) schischk. New Phytologist, 98, 683-690.

Ghazanfar, S. A., & Edmondson, J. R. (2013). Flora of Iraq. Vol. 5(2). Ministry of Agriculture & Kew Publishing

Hammer, O., Harper, D., & Ryan, P. (2001). PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica, 4,1–9.

Heinrichs, J., Klugmann, F., Hentschel, J., & Schneider, H. (2009). DNA taxonomy, cryptic speciation and diversification of the Neotropical African liverwort, Marchesinia brachiata (Lejeuneaceae, Porellales). Molecular Biology and Evolution, 53,113–121. https://doi.org/10.1016/j.ympev.2009.05.032

Hijmans, R. J., Cameron, S. E., Parra, J. L., Jones, P. G., & Jarvis, A. (2005). Very high resolution nterpolated climate surfaces for global land areas. The International Journal of Climatology, 25, 1965–1978. https://doi.org/10.1002/joc.1276

Karrenberg, S., Liu, X., Hallander, E., Favre, A., Herforth-Rahmé, J., & Widmer, A. (2018). Ecological divergence plays an important role in strong but complex reproductive isolation in campions (Silene). International Journal of Organic Evolution, 73, 245–26. https://doi.org/10.1111/evo.13652

Maddison, D. R., & Maddison, W. P. (2000). MacClade 4. Sunderland Sinauer Associates. Published at http://macclade.org

Melzheimer, V. (1988). Silene L. In Rechinger K (Ed.) Flora des iranischen hochlandes und der umrahmenden Gebirge (Persien, Afghanistan, Teile von West-Pakistan, Nord-Iraq, Azerbaidjan.

Mucina, L. (1982). Numerical classification and ordination of ruderal plant communities (Sisymbrietalia, nopordetalia) in the western part of Slovakia. Plant Ecology, 48, 267-275. https://doi.org/10.1007/BF00055270

O’Donnell, M. S., & Ignizio, D. A. (2012). Bioclimatic predictors for supporting ecological applications in the conterminous United States. US Geological Survey Data Series, 691(10).

Oxelman, B. (1996). RAPD patterns, nrDNA ITS sequences and morphologi-cal patterns in Silene section Sedoideae (Caryophyllaceae). Plant Systematics and Evolution, 201, 93-116.

Oxelman, B., & Lidén, M. (1995). Generic boundaries in the tribe Sileneae (Caryophyllaceae) as inferred from nuclear rDNA sequences. Taxon, 44, 525–542. https://doi.org/10.2307/1223498

Peel, M. C., Finlayson, B. L., & McMahon, T. A. (2007). Updated world map of the Köppen-Geiger climate classification. Hydrology and Earth System Sciences, 11, 1633–1644.

Peppe, D., Royer, D., Cariglino, B., Oliver, S., Newman, S., Leight, E., Enikolopov, G., Fernandez Burgos, M., Herrera, F., & Adams, J. (2011). Sensitivity of leaf size and shape to climate global patterns and paleoclimatic applications. New Phytologist, 190, 724-739. https://doi.org/10.1111/j.1469-8137.2010.03615.x

Posada, D., & Crandall, K. A. (1998). Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817 – 818.

Rautenberg, A., Sloan, D. B., Alden, V., & Oxelman, B. (2012). Phylogenetic relationships of Silene multinervia and Silene section Conoimorpha (Caryophyllaceae). Systematic Botany, 37(1), 226–237. https://doi.org/10.1600/036364412X616792

Rice W.R. (1989). Analyzing tables of statistical tests. Evolution, 43, 223–225

Ronquist, F., & Huelsenbeck, J. P. (2003). MrBayes 3 Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572– 1574. https://doi.org/10.1093/bioinformatics/btg180

Royer, D. L., Wilf, P., Janesko, D. A., Kowalski, E. A., & Dilcher, D. L. (2005). Correlations of climate and plant ecology to leaf size and shape: potential proxies for the fossil record. American Journal of Botany, 92(7), 1141-1151. https://doi.org/10.3732/ajb.92.7.1141

Safaei, M., Sheidai, M., Alijanpour, B., & Noormohammadi, Z. (2016). Species delimitation and genetic diversity analysis in Salvia with the use of ISSR molecular markers. Acta Botanica Croatica, 75(1), 45–52. https:// doi. org/ 10. 1515/ botcro- 2016- 0005

Safaeishakib, M., Assadi, M., Mehregan, I., & Ghazanfar, S. (2020). Phylogenetic study of Silene sections Auriculatae, Spergulifoliae, Ampullatae, and Lasiocalycinae in Iran. Phytotaxa, 472 (2), 169–183. https://doi.org/10.11646/phytotaxa.472.2.7

Safaeishakib, M., Assadi, M., Mehregan, I., & Ghazanfar, S. (2023). Cryptic Molecular- Geographical Divergence among Silene aucheriana Populations Report by ISSR Data. Biologia, 78 (4), https://doi.org/10.1007/s11756-023-01390-x

Sheidai, M., Gholipour, A., & Noormohammadi, Z. (2010). Species relationship in the genus Silene L. Section Auriculatae (Caryophyllaceae) Based on morphology and RAPD analysis. Acta Biologica Szegediensis, 54(1), 25-31

Swofford, (2002). PAUP*: Phylogenetic analysis using parsimony (and other methods) 4.0b10. Sunderland, Massachusetts. Sinauer Associates.

Ter Braak, C. (1986). Canonical correspondence analysis: a new eigenvector technique for multivariate direct gradient analysis. Journal of Ecology, 67, 1167- 79. https://doi.org/10.2307/1938672

Thorpe, R. (1987). Geographic variation: a synthesis of cause, data, pattern and congruence in relation to subspecies, multivariate analysis and phylogenesis. Italian Journal of Zoology, 54, 3-11.

Townsend, C., Melzheimer, V., Kandemir, A., Ghazanfar, S. A., & Haloob, A. (2016). Caryophyllaceae. In: Ghazanfar, S.A. & Edmondson, J.R. (Eds.) Flora of Iraq, vol. 5 (2). Baghdad: Ministry of Agriculture of the Republic of Iraq, pp. 6 – 123.

Verheijen, L. M., Brovkin, V., Aerts, R., & Bönisch, G. (2013). Impacts of trait variation through observed trait climate relationships on performance of an earth system model: a conceptual analysis. Biogeosciences, 10, 5497– 5515. https://doi.org/10.5194/bg-10- 5497-2013.

Živković, B., Radović, J., Sokolović, D., Šiler, B., Banjanac, T. & Štrbanović, R. (2012). Assessment of genetic diversity among alfalfa (Medicago sativa L.) genotypes by morphometry, seed storage proteins and RAPD analysis. Industrial Crops and Products, 40, 285–291. https://doi.org/10.1016/j.indcrop.2012.03.027

Downloads

Published

2023-06-26

How to Cite

Safaeishakib, M., Assadi, M., Ghazanfar, S. A., & Mehregan, I. (2023). Genetic diversity and morphological variability of Iranian Silene aucheriana populations inferred from nrDNA ITS sequences and morphological analysis. Hacquetia, 23(1), 151–164. Retrieved from https://ojs.zrc-sazu.si/hacquetia/article/view/10869

Issue

Section

Articles